水稻抽穗期遗传与分子调控机理研究进展

doi:10.3969/j.issn.10017216.2012.03.018

摘要/Abstract

摘要： 抽穗期是与水稻产量相关的重要农艺性状之一。介绍了水稻抽穗期的遗传方式和生长发育特性；总结了618个定位的抽穗期QTL和以Hd1、Hd3a、Ghd7、Ehd1等为代表的控制水稻抽穗期的重要功能基因，并初步形成了水稻抽穗期的调控网络。还进一步讨论了水稻抽穗期研究中所面临的问题以及其在生产实践中的应用前景。

关键词: 抽穗期, 数量性状基因座, 基因, 水稻

Abstract: Heading date is one of the most important agronomic traits closely related to rice yield in rice.The genetic pattern, growth and development characters of heading date in rice were reviewed. A total of 618 rice QTLs mapped and some important functional genes, such as Hd1, Hd3a, Ghd7, Ehd1 etc, were involved in control of the heading date. The regulation network of these headingdate genes has been preliminary established. The challenge of heading date mechanism research in rice and their prospects of applications in practice were dicussed.

Key words: heading date, quantity trait loci, gene, rice

中图分类号:

胡时开,苏岩,叶卫军,郭龙彪*. 水稻抽穗期遗传与分子调控机理研究进展[J]. 中国水稻科学, 2012, 26(3): 373-382.

HU Shikai, SU Yan, YE Weijun, GUO Longbiao*. Advances in Genetic Analysis and Molecular Regulation Mechanism of Heading Date in Rice (Oryza sativa L.)[J]. Chinese Journal of Rice Science, 2012, 26(3): 373-382.

参考文献

\[1\]崔读昌. 气候变暖对水稻生育期影响的情景分析. 应用气象学报, 1995(3): 361365.

\[2\]陈恩谦. 不同类型水稻品种营养生长期的温光效应研究. 中国农学通报, 2005, 21(5): 242244.

\[3\]陈恩谦. 温光生态因素对不同类型水稻幼穗分化期长短的影响. 中国农学通报, 2005(9): 203205.

\[4\]陈恩谦. 对不同类型水稻品种营养生长期的温光效应研究. 种子, 2007, 26(5): 7274.

\[5\]Li Z, Pinson S R M, Stansel J W, et al. Identification of quantitative trait loci (QTLs) for heading date and plant height in cultivated rice (Oryza sativa L.) . Theor Appl Genet,   1995, 91(2): 374381.

\[6\]Lu C, Shen L, Tan Z, et al. Comparative mapping of QTLs for agronomic traits of rice across environments using a doubled haploid population. Theor Appl Genet,   1996, 93(8): 12111217.

\[7\]谭震波, 沈利爽, 况浩池, 等. 水稻上部节间长度等数量性状基因的定位及其遗传效应分析. 遗传学报, 1996(6): 439446.

\[8\]林鸿宣, 钱惠荣, 熊振民, 等. 几个水稻品种抽穗期主效基因与微效基因的定位研究. 遗传学报, 1996(3): 205213.

\[9\]郭龙彪, 罗利军, 邢永忠, 等. 汕优63重组自交系群体重要农艺性状遗传分析和利用. 作物学报, 2002, 28(5): 644649.

\[10\]Li Z K, Yu S B, Lafitte H R, et al. QTL×environment interactions in rice：Ⅰ. Heading date and plant height. Theor Appl Genet,   2003, 108(1): 141153.

\[11\]Mei H W, Luo L J, Ying C S, et al. Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two testcross populations. Theor Appl Genet,   2003, 107(1): 89101.

\[12\]Mei H W, Li Z K, Shu Q Y, et al. Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two backcross populations. Theor Appl Genet,   2005, 110(4): 649659.

\[13\]Thomson M J, Tai T H, McClung A M, et al. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet,   2003, 107(3): 479493.

\[14\]Thomson M J, Edwards J D, Septiningsih E M, et al. Substitution mapping of dth1.1, a floweringtime quantitative trait locus (QTL) associated with transgressive variation in rice, reveals multiple subQTL. Genetics,   2006, 172(4): 25012514.

\[15\]Wang C M, Hideshi Y, Atsushi Y, et al. Identification of quantitative trait loci controlling F2 sterility and heading date in rice. Acta Genet Sin, 2002, 29(04): 339342.

\[16\]Maheswaran M, Huang N, Sreerangasamy S R, et al. Mapping quantitative trait loci associated with days to flowering and photoperiod sensitivity in rice (Oryza sativa L.) . Mol Breeding, 2000, 6(2): 145155.

\[17\]Yano M, Harushima Y, Nagamura Y, et al. Identification of quantitative trait loci controlling heading date in rice using a highdensity linkage map. Theor Appl Genet,   1997, 95: 1025 1032.

\[18\]Yano M, Katayose Y, Ashikari M, et al. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell,   2000, 12(12): 24732484.

\[19\]Takahashi Y, Shomura A, Sasaki T, et al. Hd6,   a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2. Proc Natl Acad Sci USA, 2001, 98(14): 79227927.

\[20\]Kojima S, Takahashi Y, Kobayashi Y, et al. Hd3a , a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under shortday conditions. Plant Cell Physiol, 2002, 43(10): 10961105.

\[21\]Doi K, Izawa T, Fuse T, et al. Ehd1, a Btype response regulator in rice, confers shortday promotion of flowering and controls FTlike gene expression independently of Hd1. Genes & Dev,   2004, 18(8): 926936.

\[22\]Saito H, Yuan Q, Okumoto Y, et al. Multiple alleles at Early flowering 1 locus making variation in the basic vegetative growth period in rice (Oryza sativa L.). Theor Appl Genet,   2009, 119(2): 315323.

\[23\]Matsubara K, Yamanouchi U, Wang Z X, et al. Ehd2,   a rice ortholog of the maize INDETERMINATE1 gene, promotes flowering by upregulating Ehd1. Plant Physiol,   2008, 148(3): 14251435.

\[24\]Izawa T, Oikawa T, Tokutomi S, et al. Phytochromes confer the photoperiodic control of flowering in rice. Plant J, 2000, 22(5): 391399.

\[25\]Andrés F， Galbraith D W，Talón M，et al. Analysis of PHOTOPERIOD SENSITIVITY5 sheds light on the role of phytochromes in photoperiodic flowering in rice. Plant Physiol,   2009, 151(2): 681690.

\[26\]Saito H, Okumoto Y, Yoshitake Y, et al. Complete loss of photoperiodic response in the rice mutant line X61 is caused by deficiency of phytochrome chromophore biosynthesis gene. Theor Appl Genet,   2011, 122(1): 109118.

\[27\]Xue W, Xing Y, Weng X, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet,   2008, 40(6): 761767.

\[28\]Wei X, Xu J, Guo H, et al. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol, 2010, 153(4): 17471758.

\[29\]Yan W H, Wang P, Chen H X, et al. A major QTL,   Ghd8 ,   plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice. Mol Plant,   2011, 4(2): 319330.

\[30\]Dai C, Xue H W. Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling. EMBO J,   2010, 29(11): 19161927.

\[31\]Hayama R, Yokoi S, Tamaki S, et al. Adaptation of photoperiodic control pathways produces shortday flowering in rice. Nature,   2003, 422(6933): 719722.

\[32\]Kim S L, Lee S, Kim H J, et al. OsMADS51 is a shortday flowering promoter that functions upstream of Ehd1, OsMADS14, and Hd3a. Plant Physiol,   2007, 145(4): 14841494.

\[33\]Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUSLIKE 20 (SOC1/AGL20) ortholog in rice. Plant J,   2004, 38(5): 754764.

\[34\]Colasanti J, Tremblay R, Wong A, et al. The maize INDETERMINATE1 flowering time regulator defines a highly conserved zinc finger protein family in higher plants. BMC Genom,   2006, 7(1): 158.

\[35\]Park S J, Kim S L, Lee S, et al. Rice Indeterminate 1 (OsId1) is necessary for the expression of Ehd1 (Early heading date 1) regardless of photoperiod. Plant J,   2008, 56(6): 10181029.

\[36\]Wu C, You C, Li C, et al. RID1,   encoding a Cys2/His2type zinc finger transcription factor, acts as a master switch from vegetative to floral development in rice. Proc Natl Acad Sci USA,   2008, 105(35): 1291512920.

\[37\]McClung C R. Plant circadian rhythms. Plant Cell,   2006, 18(4): 792803.

\[38\] Itoh H, Nonoue Y, Yano M, et al. A pair of floral regulators sets critical day length for Hd3a florigen expression in rice. Nat Genet,   2010, 42(7): 635638.

\[39\]Komiya R, Ikegami A, Tamaki S, et al. Hd3aand RFT1 are essential for flowering in rice. Development,   2008, 135(4): 767774.

\[40\]Tamaki S, Matsuo S, Wong H L, et al. Hd3a protein is a mobile flowering signal in rice. Science,   2007, 316(5827): 10331036.

\[41\]Taoka K, Ohki I, Tsuji H, et al. 1433 proteins act as intracellular receptors for rice Hd3a florigen. Nature,   2011, 476(7360):332335.

\[42\] Wuriyanghan H, Zhang B, Cao W H, et al. The ethylene receptor ETR2 delays floral transition and affects starch accumulation in rice. Plant Cell, 2009, 21:14731494.

\[43\]Kobayashi Y, Weigel D. Move on up, it’s time for change—mobile signals controlling photoperioddependent flowering. Genes & Dev,   2007, 21(19): 23712384.

\[44\]Turck F, Fornara F, Coupland G. Regulation and identity of florigen: FLOWERING LOCUS T moves center stage. Annu Rev Plant Biol,   2008, 59: 573594.

\[45\]Kardailsky I, Shukla V K, Ahn J H, et al. Activation tagging of the floral inducer FT. Science,   1999, 286(5446): 19621965.

\[46\]Samach A, Onouchi H, Gold S E, et al. Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science,   2000, 288(5471): 16131616.

\[47\]SuarezLopez P, Wheatley K, Robson F, et al. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature,   2001, 410(6832): 11161120.

\[48\]Yanovsky M J, Kay SA. Molecular basis of seasonal time measurement in Arabidopsis. Nature,   2002, 419(6904): 308312.

\[49\]Valverde F, Mouradov A, Soppe W, et al. Photoreceptor regulation of CONSTANS protein in photoperiodic flowering. Science,   2004, 303(5660): 10031006.

\[50\]Abe M, Kobayashi Y, Yamamoto S, et al. FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science,   2005, 309(5737): 10521056.

\[51\]Corbesier L, Vincent C, Jang S, et al. FT protein movement contributes to longdistance signaling in floral induction of Arabidopsis. Science,   2007, 316(5827): 10301033.

\[52\]Giakountis A, Coupland G. Phloem transport of flowering signals. Curr Opin Plant Biol,   2008, 11(6): 687694.

\[53\]Blázquez M A. Flower development pathways. J Cell Sci,   2000(113): 35473548.

\[54\]EndoHigashi N, Izawa T. Flowering time genes Heading date 1 and Early heading date 1 together control panicle development in rice. Plant Cell Physiol,   2011, 52(6): 10831094.

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